The full GFP density is shown at the top a sliced view with a fitted GFP atomic model is shown at the bottom. c Two wall-eyed stereo views of the sharpened GFP density map. b Gold-standard FSC curve for the reconstruction. a A density map obtained following multi-body refinement in RELION is shown colored by local resolution in a side view (left) and a sliced view (right), showing the central helix in the center of the GFP beta barrel. Near-atomic resolution map of the DARPin adaptor and its bound cargo protein GFP.
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